期刊:JOURNAL OF INSECT SCIENCE ISSN:1536-2442 , 年:2017 . 卷:17 . 期:2

原文链接:http://dx.doi.org/10.1093/jisesa/iex014

摘要

The oriental fruit moth, Grapholita molesta (Busck) (Lepidoptera: Tortricidae), is an important pest of most stone and pome fruits and causes serious damage to the fruit industry worldwide. This insect pest has been primarily controlled through the application of insecticides; as a result, G. molesta has developed resistance to many different types of insecticides. To identify detoxification genes, we have, de novo, sequenced the transcriptome of G. molesta (Lepidoptera: Tortricidae) and yielded 58,970 unigenes of which 26,985 unigenes matched to known proteins. In total, 2,040 simple sequence repeats have been identified. The comprehensive transcriptome data set has permitted us to identify members of important gene families related to detoxification in G. molesta, including 77 unigenes of putative cytochrome P450s, 28 of glutathione S-transferases, 46 of Carboxylesterases, and 31 of insecticide targets. Orthologs of some of these unigenes have shown to play a pivotal role in insecticide resistance in other insect species and those unigenes likely have similar functions in G. molesta.

更多>>>

 

关键词
Grapholita molesta;  transcriptome;  detoxification gene;  insecticide target;  illumina sequencing

附加关键词:
GLUTATHIONE-S-TRANSFERASE;  VECTOR ANOPHELES-GAMBIAE;  MYZUS-PERSICAE SULZER;  DROSOPHILA-MELANOGASTER;  LOCUSTA-MIGRATORIA;  DIAMONDBACK MOTH;  MICROSATELLITE MARKERS;  MALATHION RESISTANCE;  DIAMIDE INSECTICIDES;  TRIBOLIUM-CASTANEUM

作者信息

通讯作者:
     Ma, RY , Shanxi Agr Univ, Coll Agr, Taigu 030801, Shanxi, Peoples R China.
作者机构:
     [1] [ Guo, Yanqiong;  Chai, Yanping;  Zhang, Lijun;  Zhao, Zhiguo;  Ma, Ruiyan ︾ ]  Shanxi Agr Univ, Coll Agr, Taigu 030801, Shanxi, Peoples R China.
     [2] [ Gao, Ling-Ling ]  CSIRO, Agr & Food, Private Bag 5, Wembley, WA 6913, Australia.
作者邮箱:
     guoyq1979@163.com
     1606673676@qq.com
     butterflycoco@163.com
     maruiyan2004@163.com